Government Contract | Iowa
Next Generation Library Construction and Sequencing services

Bid Information

Bid Alert No: 00000524621

Bid Title: Next Generation Library Construction and Sequencing services

Agency Bid No. Title: 63331

Received Date: 03/25/2016

Close Date: 04/04/2016

Purchase Type: 2 year term

Delivery Point:

Delivery Date: Not Stated


Specifications include, but are not limited to:Iowa State University (ISU) is requesting bids to provide Next Generation Library Construction and Sequencing services, utilizing the Illumina sequencing by synthesis (SBS) process, on a two year contract basis for the Ecology, Evolution, and Organismal Biology Department. ISU estimates approximately 250 RNA samples and 50 DNA samples (including but not limited to RNA and DNA samples extracted from cotton tissues) will require library construction and subsequent sequencing on HiSeq 2500 or newer platform in the next 24 months. Library Construction: ISU requests that each prepared library be indexed for multiplexing in batches of a minimum of 10 samples, and that the service includes post-library preparation quality assurance (such as Bioanalyzer distribution analysis and qPCR quantification or equivalent QA tests). Service provider must agree to custom library insert sizes at the discretion of the project (for both DNA and RNA libraries; e.g., circa 400nt) and must indicate multiplexing capabilities. Library preparation method (e.g., Tru-seq, NEB, custom) must be specified. Sequencing on HiSeq 2500 or newer: ISU requests a minimum of approximately 270M reads per lane, sequenced as 2x150bp and with >80% of bases as Q20 or better. Service requested includes cluster generation, sequencing, and basic data clean-up (removing adaptors, contamination and low-quality reads from raw reads

Bid Related Documents

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Product Code: 90927

Agency Information

Issuing Agency: Iowa State University

State: Iowa

Agency Type: State and Local

Contact: Yi Wu

Phone: 515-294-1857




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