Government Contract | Virginia
Estuarine Sediment Sample Toxicity Tests

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Bid Information

Bid Alert No: 00000511942

Bid Title: Estuarine Sediment Sample Toxicity Tests

Agency Bid No. Title: 16-06-RB

Received Date: 03/15/2016

Close Date: 04/06/2016

Purchase Type: Term:

Delivery Point:

Delivery Date: Not Stated

Special Notices: Pre-Bid Conference Optional 03/22/2016 10:30 AM


Specifications include, but are not limited to: The Contractor shall perform the analysis of toxicity and the collection of relatedmeasurements for up to 55 estuarine sediment samples, including up to fiveadditional Quality Assurance (QA) duplicate samples, each year that the contractis in effect, as specified below:A. TOXICITY TESTING:1. Ten-day static acute toxicity tests with the marine amphipod Leptocheirusplumulosus shall be conducted for all samples in accordance with standardASTM guidelines and EPA methods (Test Method 100.4; U. S. EPA,1994).2. An estimate of up to 5 samples each year shall be collected by DEQ fromtidal freshwater sites. Such samples will be identified by the DEQ ContractAdministrator and parallel freshwater tests with Hyalella azteca will berequired (Method 100.1 Hyalella azteca 10-d Survival and Growth Test,U. S. EPA, 2000).a. DEQ will attempt to collect all such tidal freshwater samples to be runin a single batch. Study design for 2016 indicates that two suchfreshwater sites are expected.b. In either case, the test endpoint shall be control-corrected survival ofthe organisms at the end of the ten-day test.c. No growth measurements will be required.B. QUALITY ASSURANCE/QUALITY CONTROL SUBMITTAL:1. A copy of the contractors Quality Assurance/Quality Control protocolsshall be submitted to the DEQ Contract Administrator prior to the analysisof samples.a. The protocols shall conform, at a minimum, to those specified in theNational Coastal Condition Assessment: Laboratory OperationsManual (U.S. EPA, 2014a) and adhere to Quality Control guidelinesspecified in the National Coastal Condition Assessment QualityAssurance Project Plan (U.S. EPA, 2014b), both of which are availablefrom the DEQ Contract Administrator in the form of *.PDF files.b. Acceptable performance in the execution of these tests shall bedemonstrated to the DEQ Contract Administrator prior to the initiationof testing.c. At a minimum, control charts of toxicity results for reference toxicanttests (CdCl2, CuSO4 or sodium dodecyl sulfide SDS) utilizing 4Leptocheirus plumulosus shall be provided to the DEQ ContractAdministrator, to document acceptable performance.d. Similar documentation as to c. above shall be provided to the DEQContract Administrator for performance of Hyalella azteca withreference toxicants.e. The contractor shall agree to allow a QA audit of laboratory and/ordata entry procedures by an authorized agent of DEQ at any time,following advance notice.C. LABELING AND SHIPMENT INSTRUCTIONS:1. DEQ Responsibilities:a. Samples will be collected by DEQ field crews.b. Sample labeling protocols will remain identical to those previouslyused within the EPA National Coastal Condition Assessment Program.c. Sample identification will consist of a state code, followed by a yeardesignation, a dash, and a site identification number (e.g., VA16-001A)as well as a DEQ Station Name, identifying the site by a two-digitmajor basin and sub-basin code, a three-digit waterbody identificationcode, plus river-mile designation (e.g., 7BPNK003.14).d. DEQ will ship samples to the contractor such that the 30-day holdingtime will not be exceeded before performing the toxicity tests.2. Contractors Responsibilities:a. The contractor should expect to receive biweekly shipments of one toeight refrigerated samples from early July until field sampling iscompleted, usually by mid-September to early October of each yearduring which the contract is effective.b. The Contractor shall adhere to strict sample tracking procedures toensure samples are handled in an expeditious manner. Details of thetracking procedure shall include, at a minimum, immediate notificationof shipment arrival followed, within 24 hours, by an electronic listingof the samples received as well as a faxed or scanned hardcopy of thecorresponding annotated and signed sample transfer receipt (seeAppendix A). In the case of hand delivery, a sample transfer receipt,annotated and signed by the recipient, will suffice unless subsequentannotations and comments on sample condition are required, in suchcase notify DEQ Contract Administrator immediately.D. TEST RESULTS AND FINAL REPORTS1. All testing shall be completed within 30 days of receipt of the last sample.2. Results for each individual test shall be reported in an electronic format(spreadsheets) to include, at a minimum, the parameters summarized inAppendix B, accompanied by counts of individual organisms at thebeginning and surviving at the end in each replicate of each test, and asummary of physical/chemical observations (e.g., pH, DO, salinity, etc.)for each test vessel. 53. Data and the report shall be delivered to DEQ within 90 days of receipt ofthe final sample.4. All data shall be associated with the DEQ sample ID provided with thesample.5. The contractor shall provide the DEQ Contract Administrator an electroniccopy (e.g., Microsoft Word or *.PDF file) of a written final reportdetailing the methods used and the results of Quality Assurance measureswithin 90 days of receipt of the final sample.6. Any laboratory notes discussing problems encountered must be included asan appendix to D. 5. above.7. A copy of the cover letter which accompanies the results shall be sent tothe DEQ Contract Administrator.8. Original records, such as laboratory notebooks, shall be retained for atleast three years following final report submission, as copies may berequested for submission by DEQ.E. VIRGINIA ENVIRONMENTAL LABORATORY ACCREDITATIONPROGRAM (VELAP)At the present time there is apparently no NELAP/VELAP protocol for theacute toxicity testing of sediments with estuarine or marine organisms. In theevent that such a protocol is established during the period defined by thecontract, continuation and renewal of the service contract will requirecertification by the VELAP. Information relating to VELAP accreditation canbe found at Whether itis a request for primary certification or via reciprocity with a recognizedNELAP accredited authority, such certification must be established directlyfrom VELAP. In either case, fees as established by the VELAP schedule at thetime of application will apply. Accreditation must be in place prior to theinitiation of the field season, on 1 July of each calendar year the contract is ineffect. Renewal of VELAP accreditation for commercial laboratories isrequired on an annual basis.

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Product Codes: 92693, 98991

Agency Information

Issuing Agency: Commonwealth of Virginia Department of Environmental Quality

State: Virginia

Agency Type: State and Local

Contact: Rene Bishop

Phone: 804-698-4061

Fax: 804-698-4178




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